Our Approach

We seek to enter into partnerships with a focus on our customers’ needs. We enable discovery and optimization in a way you can’t find anywhere else. Through our partnerships, we leverage our technology to create disruptive and patentable solutions that meet your needs.

Gene Therapy

We help customers tune the size, strength, and specificity of their DNA package to work in the therapeutic sweet spot for their disease. This can involve reducing promoter size to fit in AAV, or adding enhancer elements to modulate strength. We can search genome wide to find novel repressors to reduce side effects.

For customers working with nanoparticles or post-mitotic cells we can help find sequences that help localize DNA to the nucleus. We uniquely offer customers the ability to combine all of these elements to maximize success of their gene therapy. We have experience in 18 different human/mouse tissue types including live in-vivo models.

Bio Manufacturing

We have a proven track record of working in production organisms. For CHO expression, we have evaluated over 5 million mutants of the CMV promoters and identified multiple positions with greater than 2x increased expressions and combination mutants with significantly more expression. We additionally have validated methods to remove GC content repeats and other elements that reduce long term stability.

In prokaryotes we have tuned bacterial expression from the T7 promoter in cells and cell-free extract as well as novel bacterial promoters from other gram-positive species. We have successfully worked with 7 different prokaryotic and eukaryotic species and have the ability to work in any species. Circularis’ technology is focused on regulatory elements, so no changes to your cell line, strain, or coding sequence.

Synthetic Biology

We optimize transcriptional controls by discovering and enhancing promoters in a variety of species and application types. We work with industrial bio partners on bacterial and yeast strains that increase production of proteins and small molecules. In multi-step pathways, we can create tunable ladders of promoter strength to better balance expression and maximize yield. Our technology works in poorly-studied species without prior knowledge by doing genome-wide searches of short or long fragments under specific conditions. Our NGS-based methods allow us to take a large number of samples to study promoter activity under different conditions and time points. We can work in whole organisms or cell cultures such as in our ag-bio projects.